P-86: Protective Effect of Vitamin C and Royal Jelly on Sex Hormones on Phenylhydrazine Treated Adult Male Mice

Background: Phenylhydrazine (PHZ) is used to induce experimental anemia in animal models. On the other hand royal jelly is known as antioxidant compound. Therefore, here in present study we aimed to evaluate the protective effect of royal jelly on PHZ-induced damages in testicular tissue and compare its impact with vitamin C. Materials and Methods: Eighteen mature male mice were randomly divided into 3 groups. In order to induce experimental anemia, PHZ was administrated at dose of 8 mg/kg, ip and then it continued as 6 mg/kg, every 48 hours, ip in all groups. The test groups subdivided into non-treated PHZ-received, royal jelly-received (100 mg/kg, orally) and vitamin C-received (250 mg/kg, ip).After treatment period, blood samples were taken and their serum was separated. Testosterone was assessed by Radioimmunoassay (RIA) method, and Lutenizing (LH) and follicle stimulating hormone (FSH) were assessed by enzyme-linked immunosorbent assay (ELISA) with special kits. The data were analyzed by SPSS (Version 20; SPSS Inc., Chicago, USA) one way ANOVA and TUKEY tests. A p value of less than 0.05 was considered significant. Results: A significant decrease in serum hormone test hormone levels (testosterone, FSH, LH) in the same control group compared with the control group and the administration of royal jelly and vitamin C serum levels of these hormones, compared with the same control group. significant increase. Conclusion: Toxicity in reproductive organ was performed by oxidative stress due to administration of Phenylhydrazine. According to toxic effect of Phenylhydrazine on hypothalamus-hypophysis- gonadic axis,this drug caused a notified reduction in sex hormones. Vitamin C and Royal Jelly as a potential antioxidant agent has been able to increase the sex hormone levels and protect reproductive system against the deleterious effects of oxidative stress due to Phenylhydrazine that usually cause infertility. Therefore, Phenylhydrazine through oxidative stress disturb the hormonal system and vitamin C and royal jelly as a nutrient antioxidant has protective role in toxic effects of this drug.